Edorium Journal of

Tumor Biology

 
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Original Article
 
Molecular analysis and clinicopathologic features of advanced colorectal cancer in Algerian patients
Kenza Boudida-Berkane1, Hind Benchaa2, Sonia Ait younes3, Hayet Ait Kaci4, Mohammed Oukkal5, Hacene Mahfouf6, Kamel Bouzid7
1University Sciences &Technology, Algiers, Algeria. Researcher in Molecular oncology laboratory Medical Oncology Department Pierre & Marie, Center Algiers, Algeria.
2University Algiers1 Medical Oncology Department Pierre & Marie Curie Center, Algiers, Algeria.
3University Algiers1, Anatomo pathology Department, Mustapha Hospital Algiers, Algeria.
4University Algiers1, Anatomo pathology Department Pierre & Marie Center, Algiers, Algeria.
5University Algiers1, Medical Oncology Department Pierre & Marie Curie Center, Algiers Algeria.
6University Algiers1, Medical Oncology Department, Rouiba Hospital, Algiers, Algeria.
7University Algiers1, Head of the Medical Oncology Department & Director of Molecular oncology laboratory, Medical Oncology Department Pierre & Marie Curie Center, Algiers, Algeria.

Article ID: 100004T09KB2016
doi:10.5348/T09-2016-4-OA-1

Address correspondence to:
Kenza Boudida-Berkane
University Sciences &Technology, Algiers
Algeria

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How to cite this article
Boudida-Berkane K, Benchaa H, Younes SA, Kaci HA, Oukkal M, Mahfouf H, Bouzid K. Molecular analysis and clinicopathologic features of advanced colorectal cancer in Algerian patients. Edorium J Tumor Bio 2016;3:1–8.


Abstract
Aims: This retrospective study aims to analyze tumors hot spot mutations frequency in KRAS, BRAF and microsatellite instability (MSI) status of tumors in Algerian patients with advanced colorectal cancer (CRC) which can predict prognosis and contribute to decisions on treatment strategies.
Methods: KRAS exon 2, BRAF exon 15 were analyzed by direct sequencing of amplified PCR products in 102 tumors patients with advanced CRC cancer. The MSI was determined using a panel of five mononucleotide markers (BAT25, BAT26, NR21, NR22 and NR24).
Results: BRAF and KRAS mutations were detected in 4.9% and 31.3% of the tumors patients respectively. Activating mutations in codon 12 and 13 in KRAS was located in the right colon 40.6% versus 25% in the left colon. (62.5%) with KRAS mutations are well or moderately differentiated. The aminoacid changes are more frequently observed in codon 12 (29/32) than in codon 13 (3/32) and G12D (43.8%) is the most frequent mutation. BRAF v600E mutation is observed in proximal colon in 3 of 5 tumors (60%) in patients with older age > 50 years. (53.1%).BRAF wild type tumors (79%) were associated with MSI-H.
Conclusion: The results of KRAS and BRAF mutation analysis could be used in the selection of Algerian patients with CRC for anti epidermal growth factor receptor (anti-EGFR) therapy and MSI-H status associated with BRAF wild type (Wt) may be suggesting the possible presence of Hereditary non polyposis colorectal cancer (HNPCC) syndrome.

Keywords: Algeria, BRAF and KRAS mutations, Microsatellite instability (MSI), Colorectal cancer (CRC), Non polyposis colorectal cancer


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Author Contributions:
Kenza Boudida-Berkane – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Hind Benchaa – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Sonia Ait Younes – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Hayet Ait Kaci – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Mohammed Oukkal – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Hacene Mahfouf – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Kamel Bouzid – Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of submission
The corresponding author is the guarantor of submission.
Source of support
None
Conflict of interest
Authors declare no conflict of interest.
Copyright
© 2016 Kenza Boudida-Berkane et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.



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